cryopreserved primary human hepatocytes (phh) Search Results


normal hepatocyte cell line thle 3  (ATCC)
96
ATCC normal hepatocyte cell line thle 3
Normal Hepatocyte Cell Line Thle 3, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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thiazole 5 carbaldehydeo  (Thermo Fisher)
99
Thermo Fisher thiazole 5 carbaldehydeo
Thiazole 5 Carbaldehydeo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantikine human hgf elisa kit  (R&D Systems)
95
R&D Systems quantikine human hgf elisa kit
Figure 6. Significant upregulation of human <t>HGF</t> in conditioned medium was confirmed by <t>ELISA.</t> HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.
Quantikine Human Hgf Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human hgf  (Mitsubishi Tanabe)
90
Mitsubishi Tanabe recombinant human hgf
Figure 6. Significant upregulation of human <t>HGF</t> in conditioned medium was confirmed by <t>ELISA.</t> HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.
Recombinant Human Hgf, supplied by Mitsubishi Tanabe, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal mouse anti-human hepatocyte  (Agilent technologies)
90
Agilent technologies monoclonal mouse anti-human hepatocyte
Figure 6. Significant upregulation of human <t>HGF</t> in conditioned medium was confirmed by <t>ELISA.</t> HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.
Monoclonal Mouse Anti Human Hepatocyte, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cryopreserved human hepatocytes  (Celsis In Vitro Inc)
90
Celsis In Vitro Inc cryopreserved human hepatocytes
Figure 6. Significant upregulation of human <t>HGF</t> in conditioned medium was confirmed by <t>ELISA.</t> HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.
Cryopreserved Human Hepatocytes, supplied by Celsis In Vitro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated goat anti human hgf antibody  (R&D Systems)
93
R&D Systems biotinylated goat anti human hgf antibody
Figure 6. Significant upregulation of human <t>HGF</t> in conditioned medium was confirmed by <t>ELISA.</t> HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.
Biotinylated Goat Anti Human Hgf Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant hgf  (R&D Systems)
96
R&D Systems recombinant hgf
Figure 6. Significant upregulation of human <t>HGF</t> in conditioned medium was confirmed by <t>ELISA.</t> HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.
Recombinant Hgf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human hgf five amino acid-deleted type  (Kringle Pharma Inc)
90
Kringle Pharma Inc recombinant human hgf five amino acid-deleted type
Figure 6. Significant upregulation of human <t>HGF</t> in conditioned medium was confirmed by <t>ELISA.</t> HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.
Recombinant Human Hgf Five Amino Acid Deleted Type, supplied by Kringle Pharma Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human recombinant hgf/sf  (PeproTech)
90
PeproTech human recombinant hgf/sf
Figure 6. Significant upregulation of human <t>HGF</t> in conditioned medium was confirmed by <t>ELISA.</t> HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.
Human Recombinant Hgf/Sf, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp il6st hs00174360 m1  (Thermo Fisher)
96
Thermo Fisher gene exp il6st hs00174360 m1
Figure 6. Significant upregulation of human <t>HGF</t> in conditioned medium was confirmed by <t>ELISA.</t> HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.
Gene Exp Il6st Hs00174360 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 6  (R&D Systems)
92
R&D Systems il 6
Figure 6. Significant upregulation of human <t>HGF</t> in conditioned medium was confirmed by <t>ELISA.</t> HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.
Il 6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 6. Significant upregulation of human HGF in conditioned medium was confirmed by ELISA. HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.

Journal: Stem cells (Dayton, Ohio)

Article Title: Nuclear fusion-independent smooth muscle differentiation of human adipose-derived stem cells induced by a smooth muscle environment.

doi: 10.1002/stem.1023

Figure Lengend Snippet: Figure 6. Significant upregulation of human HGF in conditioned medium was confirmed by ELISA. HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.

Article Snippet: Test and control media (DMEM or SMIM media exposed to hASC at time 0) were collected and examined for hepatocyte growth factor (HGF) levels using the Quantikine Human HGF ELISA kit (R&D Systems, Minneapolis, MN, www.rndsystems. com) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Staining, Injection, Expressing