Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: Effects of AuNPs + radiation combined treatment on the cell proliferation of normal hepatocytes and hepatic stellate cell line. (A) MTT assays were performed using HH cells treated with AuNPs or X-ray irradiation. (B) Transmission electron microscopy images of AuNPs uptake inside the cell. (C) MTT assays were performed using hepatocyte cells (HH: left panel) and hepatic stellate cell line (LX-2: right panel) treated with AuNPs or X-ray irradiation, or a combination of the two treatments. The blue arrows indicate the magnification of AuNPs. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; HH, human hepatocyte cell.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Irradiation, Transmission Assay, Electron Microscopy

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: Cell SF of AuNPs + radiation combined treatment of normal hepatocytes. Clonogenic assay of hepatocytes after treatment with AuNPs or X-ray irradiation or a combination of the two treatments. SF, survival fraction; IR, irradiation.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Clonogenic Assay, Irradiation

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: Effects on apoptosis of AuNPs + radiation combined treatment of hepatocytes. (A) Cells were treated with PI at 48 h post-treatment after each indicated treatment and analyzed by FACS. (B) The indicated antibodies were used for western blotting of hepatocytes treated with AuNPs or X-ray irradiation or a combination of the two treatments. Values represent the means of 3 experiments ± SD. (C) Cells were treated with AuNPs and/or X-ray irradiation or a combination of the two treatments for 24 h. The cell-cycle distribution of subG1 was analyzed quantitatively. *, P<0.05 and **, P<0.01. IR, irradiation; PARP, poly (ADP-ribose) polymerase; PI, propidium iodide; FACS, fluorescence-activated cell sorting.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Western Blot, Irradiation, Fluorescence, FACS

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: IL-8 concentrations secreted from normal hepatocytes after AuNPs + X-ray irradiation combined treatment. In all therapeutic groups, a decrease in the amount of IL-8 was observed in the radiation alone group. The combination therapy did not lead to significantly lower levels of IL-8 than radiation alone. IL-8, interleukin-8; IR, irradiation.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Irradiation

Journal: Journal of Cellular and Molecular Medicine

Article Title: Human liver stem cell-derived microvesicles accelerate hepatic regeneration in hepatectomized rats

doi: 10.1111/j.1582-4934.2009.00860.x

Figure Lengend Snippet: Cytofluorimetric characterization of HLSC-derived MVs and their incorporation into hepatocytes. (A) Representative FACS analyses of MVs showing the size (with 1-, 2- and 4-μm beads used as internal size standards) and the expression of α 4 -integrin, α 5 -integrin, α 6 -integrin, CD29, CD44 and HLA-class I (dark lines) surface molecules. Dot lines indicate the isotypic controls. Ten different MV preparations were analysed with similar results. In the α 4 -integrin, CD29, CD44 experiments the Kolmogrov–Smirnov statistical analyses between relevant antibodies and the isotypic control was significant ( P < 0.001). No significant expression of α 5 -integrin, α 6 -integrin and HLA class I was observed. (B) Western blot analysis of two different MV preparations showing the expression of Staufen 2 (Stau 2) ribonucleoprotein. (C) Representative confocal microscopy micrographs of internalization by human hepatocytes (3 hrs at 37°C) of MVs labelled with red fluorescent PKH26. Where indicated MVs were pre-incubated trypsin (0.5 mM; Try-MV), with 1 μg/ml blocking monoclonal antibodies against CD29, α 4 - and α 6 -integrin or with 100 μg/ml of sHA to block CD44. Three experiments were performed with similar results. Original magnification: ×630. (D) Representative FACS analyses of internalization by hepatocytes of MVs labelled with PKH26 (black areas) pre-incubated with trypsin (0.5 mM; Try-MV) or with 1 μg/ml blocking monoclonal antibodies against CD29, α 4 - and α 6 -integrin or sHA. Black areas indicate the internalization of untreated MVs. In the first panel white area indicates the negative control (cells incubated with vehicle alone). In the other panels white areas indicate internalization of MVs after incubation trypsin or with blocking antibodies or sHA. Three experiments were performed with similar results.

Article Snippet: Human hepatocytes (Lonza) were cultured in William’s medium supplemented with 10% of FCS, 7.5 μg/ml hydrocortisone, 200 μg/ml streptomycin and 200 U/ml penicillin.

Techniques: Derivative Assay, Expressing, Control, Western Blot, Confocal Microscopy, Incubation, Blocking Assay, Bioprocessing, Negative Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Human liver stem cell-derived microvesicles accelerate hepatic regeneration in hepatectomized rats

doi: 10.1111/j.1582-4934.2009.00860.x

Figure Lengend Snippet: Proliferative and anti-apoptotic effects of HLSC-derived MVs on human hepatocytes. (A) 10 μM BrdU was added to 50,000 cells/well into 48-well plates incubated for 48 hrs in William’s E medium deprived of FCS in the presence of vehicle alone (Ctrl; white bar) or of MVs (5 μg/ml grey bar; 30 μg/ml dark bar) or of RNase-treated MVs (shaded bar). Results are expressed as mean ± S.D. of three different experiments performed in triplicate. Analyses of variance with Newmann–Keuls multicomparison test was performed; * P < 0.05 MV versus vehicle alone (Ctrl); ¦ P < 0.05 MV treated with RNase versus MV untreated. (B) The percentage of apoptotic cells was evaluated by the TUNEL assay. We used, as apoptotic stimulus, HLSC incubated with 5 mM GalN for 24 hrs in DMEM supplemented with 2% FCS. Controls (white bars) included hepatocytes incubated with vehicle alone (Ctrl) and hepatocytes incubated with vehicle containing RNase submitted to the same procedure of inactivation as in samples of RNase-treated MVs as described in ‘Material and methods’ (RNase alone); hepatocytes incubated with 5, 15, 30 μg/ml MVs (black bars); hepatocytes incubated with RNase-treated MVs (grey bar). Results are expressed as mean ± S.D. of three different experiments performed in duplicate. Analyses of variance with Newmann–Keuls multicomparison test was performed; * P < 0.05 MV versus vehicle alone; ¦ P < 0.05 MV RNase treated versus MV untreated.

Article Snippet: Human hepatocytes (Lonza) were cultured in William’s medium supplemented with 10% of FCS, 7.5 μg/ml hydrocortisone, 200 μg/ml streptomycin and 200 U/ml penicillin.

Techniques: Derivative Assay, Incubation, TUNEL Assay